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Antibody to oxidized low-density lipoprotein inhibits THP1 cells from apoptosis by suppressing NF-κB pathway activation

   
@article{CDT28600,
	author = {Rui Dai and Jing Dong and Weijuan Li and Yi Zhou and Wei Zhou and Wenping Zhou and Manhua Chen},
	title = {Antibody to oxidized low-density lipoprotein inhibits THP1 cells from apoptosis by suppressing NF-κB pathway activation},
	journal = {Cardiovascular Diagnosis and Therapy},
	volume = {9},
	number = {4},
	year = {2019},
	keywords = {},
	abstract = {Background: This study aimed to investigate whether the oxidized low-density lipoprotein (Ox-LDL) antibody is able to inhibit THP1 cell apoptosis by suppressing NF-κB pathway.
Methods: THP1 cells were induced to macrophages with phorbol 12-myristate 13-acetate (PMA). Macrophages were divided into control group, Ox-LDL group and antibody group, cells in which were treated with phosphate buffered saline (PBS), Ox-LDL (50 mg/mL), Ox-LDL (50 μg/mL) plus Ox-LDL antibody (100 mg/L), respectively, for 24 h. The apoptosis rate was determined by inverted microscopy and  ow cytometry. The protein and mRNA expression of NF-κB (P65), caspase-3 and BCL2 was detected by Western blotting and reverse transcription polymerase chain reaction (RT-PCR), respectively.
Results: The apoptosis rate reduced signi cantly in antibody group as compared to Ox-LDL and control groups (P},
	issn = {2223-3660},	url = {https://cdt.amegroups.org/article/view/28600}
}